A centrifuge is needed to clear protein expression.
It is often said that the devil is in the details, especially in complex experiments such as purification of protein expression.
A small operational error may directly affect the final result.
Although it can be roughly divided into three steps, expression, separation and purification, the process is very complicated.
There are a series of complex processes in the early stage of protein expression, which take a long time. At the last step, we should not relax our vigilance.
Protein purification is the last step in the customs clearance. Choosing the appropriate purification method can improve the purification efficiency of the protein and make protein purification simple and convenient.
Common methods include affinity chromatography, ion exchange chromatography, gel filtration chromatography, hydrophobic chromatography, etc.