You can't wash red blood cells without a low-speed centrifuge!
Red blood cells, also known as red blood cells, are often abbreviated to RBC in routine laboratory tests. They are one of the most abundant blood cells in the blood. They are also the most important medium for carrying oxygen through blood in vertebrates, and also have immune functions.
Mature red blood cells in mammals are nucleless, which means they have lost THEIR DNA.
Red blood cells also have no mitochondria. They release energy by breaking down glucose.
You transport oxygen, you transport some carbon dioxide.
Carbon dioxide is dark purple when transported and oxygen is bright red.
Red blood cells are produced in the bone marrow.
After red blood cell aging, it is easy to cause blood vessel blockage, so it will automatically return to the deep bone marrow, which will be destroyed by white blood cells.
Or as it passes through the liver, it's broken down by macrophages into bile.
The main function of red blood cells is to transport oxygen and carbon dioxide, because red blood cells contain hemoglobin, which can bind with oxygen to carry oxygen to the tissues and organs needed by the body, and transport the carbon dioxide produced by metabolism of the tissues and organs to the lungs for excretion.
Hemoglobin is a binding protein formed by the combination of hemoglobin and globin peptide chain. It belongs to pigment protein and accounts for 32% to 36% of the total red blood cell.
Hemoglobin is composed of four peptide chains, including two -peptide chains and two -peptide chains, each of which surrounds a heme group that can carry one molecule of oxygen or one molecule of carbon dioxide.
Whole blood under aseptic conditions after low speed centrifuge firstly separated plasma and remove white blood cells, blending into the red blood cells in sterile saline water, and centrifuge to remove residual leukocytes, so repeated washing three times eventually removed more than 98% of the plasma, more than 90% of white blood cells, platelets, also in addition to the preservation of potassium, ammonia, lactic acid and other metabolites, has kept more than 70% of the red blood cells, add saline suspension.
The heteroproteins are removed to facilitate the subsequent steps of separation and purification.
It is mainly used in patients with anaphylaxis of blood transfusion, du patients with autoimmune hemolytic anemia, hyperkalemia and hepatorenal dysfunction.