Centrifuges are devices that are separated from the liquid phase by a centrifugal force. Depending on the sedimentation coefficient, mass, density, etc. of the substance, the method of using strong centrifugal force to separate, concentrate and purify the substance is called centrifugation. Centrifugation is the use of strong centrifugal force generated by the high-speed rotation of the centrifuge rotor to accelerate the sedimentation velocity of the particles in the liquid, and to separate the different sedimentation coefficient and buoyancy density of the sample.
The working principle of the centrifuge:
After the main drum starts to run normally, the feed liquid enters the inner drum (spiral body) from the feeding tube. Due to the centrifugal force field, the liquid evenly flows from the inner chamber outlet to the outer drum inner cavity, and the liquid phase is filtered. The mesh pores and the outer drum hole are discharged, and the solid phase is trapped inside the filter screen. Since the spiral body and the outer rotating drum have a certain difference speed, the solid phase is pushed by the spiral blades one by one toward the big end of the rotating drum, and is discharged from the discharging port.
9 points for the use of the centrifuge:
1. Do not use in places with damp, high temperature and dust.
2. To ensure safety and centrifugal effects, the instrument must be placed on a sturdy, shock-proof, level surface and ensure that the four feet are balanced.
3. Centrifugal tube dosing is as uniform as possible. If the difference is too large, large vibration will occur. At this time, stop the inspection and make the liquid meet the requirements. The centrifuge tube must be placed in even number of symmetry. The nut connecting the rotor to the motor shaft must be tightened.
4. Do not move the centrifuge during operation. Do not open the door.
5. Do not open the door when the centrifuge motor and rotor are not completely stopped.
6. The power supply has an independent ground line. It is strictly forbidden to share the neutral line and the ground line to prevent electric shock and injury.
7. After the separation is completed, the instrument should be wiped clean in time, and the power switch should be turned off and the power supply turned off.
8. Care should be taken when removing the rotor. The correct method is to use a non-metallic tool (such as a screwdriver handle) to extend the rotor body from multiple places under the rotor and repeat it until it is between the loose rotor body and the motor shaft. connection. Do not loosen the nut directly after pulling the nut, so as not to damage the flexible support of the motor.
9. Always check the rotor and centrifuge tube for cracks, aging, etc. If necessary, replace them in time.
The centrifuge is an instrument for separating samples. It is widely used in biomedicine, petrochemical, agricultural, food hygiene and other fields. It uses the difference in precipitation speed of different substances in the centrifugal force field to realize the analysis and separation of samples. The centrifuge is an indispensable experimental equipment for the sample preparation process. Proper use and maintenance can ensure the smooth progress of the experiment and improve the efficiency of the laboratory.